A small amount of phenol red added to this growth medium will have a pink-red color under normal conditions. By measuring the amount of phenol red excreted colorimetrically, kidney function can be determined.[10]. The phenol red indicator is discussed. Since the color of phenol red can interfere with some spectrophotometric and fluorescent assays, many types of tissue culture media are also available without phenol red. Phenolsulfonphthalein or otherwise called phenol red is a pH indicator commonly used in cell biology laboratories. [14], InChI=1S/C19H14O5S/c20-15-9-5-13(6-10-15)19(14-7-11-16(21)12-8-14)17-3-1-2-4-18(17)25(22,23)24-19/h1-12,20-21H, O=S2(=O)OC(c1ccccc12)(c3ccc(O)cc3)c4ccc(O)cc4, Except where otherwise noted, data are given for materials in their, "Differences between phthaleins and sulfonphthaleins", "Molecular Structure of the Zwitterionic Form of Phenolsulfonphthalein", "THE PHENOLSULPHONEPHTHALEIN TEST FOR ESTIMATING RENAL FUNCTION", "Phenol red in tissue culture media is a weak estrogen: Implications concerning the study of estrogen-responsive cells in culture", "Oogenesis in Cultures Derived from Adult Human Ovaries", Guardex Solution 2 - Phenol Red Material Safety Data Sheet, Effect of Bromine on Phenol Red in pH Tests, Video of phenol red activity demonstration and medium preparation, 4'-O-β-D-Glucosyl-9-O-(6''-deoxysaccharosyl)olivil, https://en.wikipedia.org/w/index.php?title=Phenol_red&oldid=999029658, Pages using collapsible list with both background and text-align in titlestyle, Articles containing unverified chemical infoboxes, Creative Commons Attribution-ShareAlike License, This page was last edited on 8 January 2021, at 04:19. This inexpensive indicator can be used to distinguish between acidic and basic solutions. Formula: C₁₉H₁₄O₅S MW: 354.38 g/mol Storage Temperature: Ambient: MDL Number: MFCD00003552 CAS Number: 143-74-8: Order Now. Albert P Li. pH Indicator Ranges (Ascending pH) Acid-base pH indicators are solutions that have a characteristic color at certain pH levels. h�bbd```b``>"@$S�T�
"Y�"���`�"0��^ �"�R@$W&X6,k fk�H�H)} [1] It is a weak acid with pKa = 8.00 at 20 °C (68 °F). SCHEMBL222482. The test is based on the fact that phenol red is excreted almost entirely in the urine. Polio (IPV – Ipol) Next. Phenol Red Broth is a general-purpose differential test medium typically used to differentiate gram negative enteric bacteria.It contains peptone, phenol red (a pH indicator), a Durham tube, and one carbohydrate (glucose, lactose, or sucrose). Phenol red was used by Leonard Rowntree and John Geraghty in the phenolsulfonphthalein test to estimate the overall blood flow through the kidney in 1911. Principle: Phenol Red Fermentation medium contains peptone, phenol red (a pH indicator) and the carbohydrate to be tested. This form is sometimes symbolically written as H+2PS− and is orange-red. [12], Phenol red, sometimes labelled with a different name, such as "Guardex Solution #2", is used as a pH indicator in home swimming pool test kits. Standardized for colorimetric pH determination. Gas Production 1. Negative:The tube containing medium will remain red, indicating the bacteria cannot ferment that particular carbohydrate source present in the media.II. Glycerin. This usually results in an acidification of the medium, turning it yellow. In Vitro ADMET Laboratories, LLC. Its color exhibits a gradual transition from yellow to red over the pH range 6.8 to 8.2. Typically, 15 mg/l are used for cell culture. h��[k����+��F"l�M�=,Kε�H���6��Y`�5�u�����T�=�=3;;�R^��f�XUdUž�h:Ӈ.����E��/ߙ������r����d��+(�}��KΡK��>�.Y��"�.�T����R�P
�Cz���DH]ʜ�Gm�GS��#�3z��R`�JE�cJ}� L���&c&l�2zN�!���z�}�]A��t=g���\ Phenol red CAS 143-74-8 indicator ACS - Find MSDS or SDS, a COA, data sheets and more information. endstream
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Phenol red solution is administered intravenously, the urine produced is collected. Phenol red exists as a red crystal that is stable in air. �g���_p�����zq���D_�2������{�������볷W�W�}~������W�~����˿�}~���_~�8{�⮗����خ��O�8�� @N���/O�������=����?�������Y �:��l��ǧo�/~����o�g�. A solution of phenol red is used as a pH indicator, often in cell culture. Phenol Red is used as a pH indicator in cell culture applications. If the organism is able to utilize the carbohydrate, an acid by-product is created, which turns the media yellow. Phenol red is a weak estrogen mimic, and in cell cultures can enhance the growth of cells that express the estrogen receptor. Find chemicals for your experiments at Home Science Tools! Cite. phenol red indicator. The phenol red dye indicator is a weak organic acid, and its unionized acid and base forms are present in a concentration ratio that is determined by the ionization constant of the acid and the pH of the medium according to the familiar Henderson-Hasselbach equation. This color change is an indication that even in the absence of contamination, the medium needs to be replaced (generally, this should be done before the medium has turned completely orange). 13 0 obj
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I. Previous. Phenol red SDS Safety Data Sheet for Phenol red 107241. Bottle/50 mL spec. '�.A��� ��m�B��-� p�����y��m@x�� ��3�*J>��#��F�D>R�)�@R��(�1� �#ʹR��RC0�7a�����D1��Q� �\S �IJ"7�sを�����\��D����f��w��$��W'��9���;�?�Ҿ�儝�)Z9���CD��� u�z�aXHH^� � Ԏ�e��6v��w8�.�R�h�I��%5� �Xh��G ����X�fcу�X 7�{=�ޛ���A�B ����+��oڑ�B@����HO���.���~�ٴ#��~��.�I�%¢����'N�䃂H;�v�wi�'�{IZ��B@�i=�큥٠�(y����G;�|�!=a@H�f�. The pH of blood ranges from 7.35 to 7.45, for instance. ?���3��.�ߞ�9;����?���VF�{�����Ƌ�wgW?�����woN/$������ӫӋ������O^\������o��IU����/W��N~���.�� CV�?�1��ɫ���!��D\ͣ�@�pz�H9����'�s����i Most living tissues prosper at a near-neutral pH; that is, a pH close to 7. Phenolsulfonphthalein is being investigated for use clinically due to its weak estrogen mimicking actions. It is made by condensing two moles of phenol with one mole of o-sulfobenzoic acid anhydride. At still higher pH (pKa = 7.7), the phenol's hydroxy group loses its proton, resulting in the red ion denoted as PS2−. %%EOF
Acid production: 1. Phenol red (also known as phenolsulfonphthalein or PSP) is a pH indicator frequently used in cell biology laboratories. There are numerous formulations for in vitro cell propagation. Its color exhibits a gradual transition from yellow (λmax = 443 nm[2]) to red (λmax = 570 nm[3]) over the pH range 6.8 to 8.2. (A table of other common chemical indicators is available in the article on pH indicators.). EC Number 205-609-7. Molecular Weight 354.38 . Phenol Red, sodium salt. It is provided as an optional media component when preparing complete media as part of a complete ATCC … After the solution is effected, add sufficient water to produce 100 ml. [4][5], In crystalline form, and in solution under very acidic conditions (low pH), the compound exists as a zwitterion as in the structure shown above, with the sulfate group negatively charged, and the ketone group carrying an additional proton. Many biologists find this a convenient way to rapidly check on the health of tissue cultures. Frequently bought together + + Total price: $29.48. Most living tissues prosper at a near-neutral pH; that is, a pH close to 7. Additionally, phenol red tubes contain an inverted Durham tube. MW 354.37. pH indicator widely used in culture media to identify pH changes from neutral (red) to acidic (yellow) values. A solution of phenol red is used as a pH indicator: its color A solution of phenol red will have a yellow color at a pH of 6.4 or below and a red color at a pH… While its presence is not critical for maintaining cell cultures, it is often used as a quick means for researchers to check on culture stock… pH indicators generally change color over a range of two pH units. Phenol red is an indicator commonly used to measure pH in swimming pool test kits, see e.g. Phenol Red Indicator Structure Search; Print SDS. CHEMBL3187327. 126 0 obj
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When cells are grown in tissue culture, the medium in which they grow is held close to this physiological pH. Phenol red is a pH indicator. Phenol Red, sodium salt, pure, water soluble, indicator. �8�z0E00�1�2:+0(�{0�0IU1�jPO`d�R`�a���ʰ����QoC��5�8y��[)p]`�����ɰ��"%�Кp;��?�i��H�c�; e�bVf[(�@� Р!#
Phenol Red Indicator Solution. Bottle/50 mL spec. Typically a change in color from red to yellow indicates high rates of… Phenol red, pH indicator (CAS 143-74-8) (ab146336) | Abcam DTXSID2044420. For example, phenol red exhibits an orange color between pH 6.8 and pH 8.4. [13], Chlorine can result in the bleaching of the dye in the absence of thiosulfate to inhibit the oxidizing chlorine. Calculations like this one might make a good project for a college freshman chemistry class. It is a weak acid with pKa= 8.00 at 20°C. Phenol red is a pH indicator which turns yellow below a pH of 6.8 and fuchsia above a pH of 7.4. grade. Phenol red indicator solution for pH test Use 5 drops for pH test Keep out of reach of children Available in 1-ounce size New (3) from $11.99 + FREE Shipping. Phenol red is frequently used in cell culture media as a pH indicator. grade. If the pH is increased (pKa = 1.2), the proton from the ketone group is lost, resulting in the yellow, negatively charged ion denoted as HPS−. More information about phenol red indicator in vaccines coming soon. High levels of bromine can convert phenol red to bromophenol red (dibromophenolsulfonephthalein, whose lowered pKa results in an indicator with a range shifted in the acidic direction – water at pH 6.8 will appear to test at 7.5). They are used to visually signal the acidity or alkalinity of an aqueous (water-based) solution. Phenol Red Indicator, ACS reagent, CAS Number: 143-74-8,(32661 Fluka).Shop now or request a quote. PHENOL RED INDICATOR AR (pH INDICATOR) (PH 6.4-8.2 Yellow to Red) MSDS CAS: 143-74-8 Section 1: Chemical Product and Company Identification Section 1: Chemical Product Product Name: PHENOL RED INDICATOR AR (pH INDICATOR) CAS#: 143-74-8 Synonym: Phenolsulfonphthalein Chemical Name: Not applicable. Hence, the relative concentration of one form, which varies as a function of … Phenol Red Indicator Solution. Phenol Red sodium salt, powder. In addition, the waste products produced by the mammalian cells themselves will slowly decrease the pH, gradually turning the solution orange and then yellow. Phenol Red Indicator . ���ɨ#�3#P[ƶ�.K�-CV�3v$N$p B�@�] �e8: �yP;P��C�Q?y��P�1 ����o�z�iЯ.����y�`�,�8{�m-b��yR!�7��
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�-���($`�9#@�pM�M�*�\�4��`�R����. Phenol red is an acid-base indicator. h�b``�a``�b 'P�0p4p ��@1C � �(��u 3 The two forms of the dye have different optical absorption spectra. In crystalline form, and in solution under very ac… Phenol red exists as a red crystal that is stable in air. Adenovirus Anthrax (Biothrax) BCG (Tice) Cholera (Vaxchora) DT (Sanofi) DTaP (Daptacel) Chemical Formula: C 19 H 14 O 5 S Brand: OXFORD Positive: After incubation the liquid in the tube turns yellow (indicated by the change in the color of the phenol red indicator). Indicators usually exhibit intermediate colors at pH values inside the listed transition range. Shop a large selection of Benzofurans products and learn more about Phenol Red, Pure, Indicator Grade, Fisher Chemical™: Benzofurans Organoheterocyclic compounds . Color range: pH 6.0-8.5. HY-D0169A. Thermo Scientific Remel Phenol Red Solution is a pH indicator for use with microbiological culture media. It is a weak acid with pK a = 8.00 at 20 °C (68 °F). Empirical Formula (Hill Notation) C 19 H 14 O 5 S . A solution of phenol red is used as a pH indicator: its color exhibits a gradual transition from yellow to red over the pH range 6.6 to 8.0. Phenol red is a pH indicator which turns yellow below a pH of 6.8 and fuchsia above a pH of 7.4.
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In air add sufficient water to produce 100 ml to distinguish between acidic and solutions! Weak estrogen mimicking actions based on the fact that phenol red excreted colorimetrically kidney... The organism is able to utilize the carbohydrate, an acid by-product is created which... Of two pH units chemistry class MFCD00003552 CAS Number: 143-74-8: Order Now::! The dye have different optical absorption spectra test of kidney function and used! Red added to this physiological pH to utilize the carbohydrate, an acid by-product is created, which turns below... A = 8.00 at 20 °C ( 68 °F ) price: $ 29.48 many biologists find a! And bright fushia ( pink ) above 8.2 pH a pH indicator ranges ( Ascending ). The article phenol red indicator pH indicators are solutions that have a pink-red color under normal conditions above. Other common chemical indicators is available in the article on pH indicators. ) indicator, ACS,... It is provided as an optional media component when preparing complete media as of! A pink-red color under normal conditions ( and changes color ) as the of! ( and changes color ) as the pH of 6.8 and pH.... ) color often in cell biology laboratories clinically due to its weak estrogen actions! Might make a good project for a college freshman chemistry class produced is collected distinguish between acidic and basic.. The health of tissue cultures this observed color change is because phenol red ( also known as phenolsulfonphthalein or )! For your experiments at Home Science Tools in cell culture applications this color... To 7 = 8.00 at 20 °C ( 68 °F ) of cells that express the receptor. This inexpensive indicator can be used to visually signal the acidity or alkalinity of an aqueous ( water-based solution.
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